Confocal scanner: Nipkow spinning disk (pinholes)
• Internal excitation changer: Automated 8 position wheel (25 mm)
• Internal dichroic changer: Automated 5 position wheel (25.7 × 36 mm)
• Internal emission changer: Automated 8 position wheel (25 mm)
• Filter sets provided: DAPI, E-GFP, Texas Red
• Operation mode: Automated confocal, wide field, bright field
• Observation: Direct confocal binocular viewing or camera port
• Detector compatibility: CCD camera – Sensicam EM, QE (Cooke);CoolSNAP™ HQ, Cascade 512B (Photometrics); ORCA ER, AG (Hamamatsu) and more
• Microscope compatibility: Most inverted fluorescence microscopes with 100% camera port
• FRAP: Aperture control – touch pad or RS232
• System control: Touch pad or Computer via RS232 interface
• Software drivers: IPLab (Scanalytics, Fairfax, VA);Metamorph® (Molecular Devices, Sunnyvale, CA) RS232 command set also available
• Size: 11(w) × 15.5(L) × 6(h) inches - 27.94(w) × 39.37(L) × 15.24(h) cm • Weight: 14.5 lbs / 6.6 kg • Power: 100 – 124V AC /12V DC •Real time confocal imaging up to 100 frames per second or more. Use of CCD camera as a detector allows greater sensitivity and better signal-to-noise ratio images. Binocular viewing of confocal image allows easy set up and color viewing.
1- CCD Detector: A wide range of application specific CCD cameras can be used with the confocal device including: Princeton Instruments, Photometrics (Evolve™ EMCCD, CoolSNAP™ ES, CoolSNAP™ HQ, Cascade™ EMCCD), Andor (iXon EMCCD, Luca EMCCD), Cooke (Sensicam QE, Sensicam EM), Hamamatsu® (ORCA ER, AG), and more.
3 - Easily Switch Between Confocal and Widefield Imaging/Viewing Modes: The CARV II Confocal Imager permits direct viewing of confocal images through a binocular eyepiece and/or through the camera for fast imaging setup. It is the only pinhole spinning disk fluorescence confocal systems which allows the user to quickly switch from confocal to wide-field viewing or recording.
4 - Automated Filter Changers: Automation of internal multi-position excitation (8 position), dichroic (5 position) and emission (8 position) filter wheels allows fast multi-dimensional confocal imaging. The automated filters reduce the reliance on multi-band pass filter sets allowing maximum light throughput and fast sequential imaging of up to five or more fluorescent probes in the same sample.
5 - FRAP Capabilities: A FRAP slit at the same focal plane as the confocal disk creates an adjustable rectangular aperture on the image. Passing high-intensity Hg/metal halide light through this slit enables controlled photobleaching of part of the sample followed by fluorescence recovery recording.
6- Microscope Compatibility: The CARV II Confocal Imager unit can be configured to most inverted and upright fluorescent microscopes converting your existing fluorescence microscope to a confocal imager.
7 - Imaging Software: A range of state of the art 3D software packages can be used for acquisition and analysis of confocal images including IPLab (Scanalytics) and Metamorph (Molecular Devices). Command sets needed to control the CARV II are available on request.
|• Multipoint scanning at 1000 scans/second.|
|•Low intensity high frequency scanning.|
|•Mercury/metal halide light source with liquid light guide delivery.|
|•Built-in automated multi-position excitation, dichroic and emission filter wheels.|
|•Adapts to most major models of fluorescence microscopes.|
|•Movable spinning disk and field aperture for selective illumination.|