The Dhyana95 uses backside-illuminated sCMOS thinned chip technology to avoid light interference from the wiring layer, thereby increasing the pixel area and improving the photoelectric conversion rate.
At 560nm, the quantum efficiency of Dhyana 95 is 95%, which a front illuminated sCMOS camera cannot match, and is comparable to the best EMCCD camera.
The Dhyana95 read noise is only 1.45 electrons(peak), therefore the signal to noise ratio is significantly better than other sCMOS cameras, and when the incident photons are>3, there is a better performance than EMCCD based cameras
The 2" array can not only adapt to more optical interfaces and deliver a greater field of view, but also results in fewer lens switches to find the area of interest on the sample.
Under very weak laser illumination (< 0.002 kW cm-2 ), Dhyana 95 camera showed comparable fluorescent intensity to the 97%QE EMCCD camera with 100 EM gain. On the other hand, Dhyana 95 had similar background fluorescence noise when comparing to the 82%QE sCMOS camera, which has 1.0e-low read noise.
STORM stochastic optical imaging microscopy is one of the most widely used and the highest resolution super-resolution methods. It requires a camera to have the performance advantages of high quantum efficiency, high frame rate and low noise in the visible range. Its spatial resolution is determined by the single molecule localization accuracy and Nyquist resolution simultaneously.
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