Collect the nasopharyngeal or oropharyngeal swab into VTM.
Collect sputum into the sterilization container.
Total nucleic acids are isolated from the specimens using commercially available viral RNAisolation kits. We recommend Qiagen’s or Roche’s extraction kits.
The extraction protocol should be performed following manufacturer's instruction or an internally validated protocol.
Target Genes(RdRp, E)
Recommended by WHO
COVID -19 Detection Kit has been developed in accordance to the WHO guideline for 'Laboratory testing of 2019 novel coronavirus
High target specificity & low false negative rate. Optimal primer and probe design facilitates minimal false positive and false negative results. RdRP and E gene have been designed to react in separate tubes,
allowing the highest accuracy of the test results.
Highest Performance Enzyme
'SuperScript III RT/Platinum Taq DNA polymerase' provides highest detection sensitivity and specificity with minimum amount of sample avoiding false positive and false negative.
RNA Positive Control
The test monitors the whole real-time RT-PCR process including reverse-transcription and amplification. DNA as positive control can verify the amplification process of DNA, but cannot confirm the reverse-transcription process from RNA to DNA.